ABSTRACT
Objective To compare the immunoregulatory effects of Radix Millettia Speciosa (RM Speciosa) and Radix Millettia Championi (RM Championi)on immunosuppressed mice.Methods Kunming mice were randomly divided into normal control group,CTX model group,LMS positive group,RM Speciosa groups and RM Championi groups (20,10 and 5 g/kg).The mice were treated respectively with drug or NS once a day for consecutive 20 days.Mice in the other groups were injected intraperitoneally with CTX at days 8,10 and 12 to establish immunosuppressed mice model except the normal group.The changes of body weight,immune organ weight,white blood cell (WBC)number,carbon particle clearance capability of macrophages and delayed type hypersensitivity (DTH)of mice in all groups were determined and compared.Results Compared with that in CTX group,the WBC number was significantly increased (P0.05).Conclusion Both RM Speciosa and RM Championi can improve the immune function of CTX-induced immunosuppressed mice,and RM Speciosa is slightly superior to RM Championi in improving specific cellular immunity.
ABSTRACT
<p><b>OBJECTIVE</b>To study the metabolism of mangiferin by human intestinal bacteria in vitro.</p><p><b>METHOD</b>Human intestinal bacteria and mangiferin were incubated under anaerobic conditions in vitro. The metabolite was separated and purified by D101 macroporous resin column and preparation high performance liquid chromatography, and its structure was identified by MS and NMR.</p><p><b>RESULT</b>After 12 h incubation with human intestinal bacteria, the content of mangiferin metabolite reached the maximum, and it was determined as 1, 3, 6, 7-tetrahydroxyxanthen by MS and NMR.</p><p><b>CONCLUSION</b>Mangiferin can be metabolized in vitro by human intestinal bacteria into its aglycone (1, 3, 6, 7-tetrahydroxyxanthen).</p>
Subject(s)
Humans , Bacteria , Metabolism , Chromatography, High Pressure Liquid , Intestines , Microbiology , Xanthones , MetabolismABSTRACT
OBJECTIVE: To establish an HPLC-UV method for the content determination of noradrenaline (NA) in plasma of spontaneous hypertension rats (SHR).METHODS: The determination was performed on C18 column with mobile phase consisted of methanol-0.15 mol?L-1 potassium dihydrogen phosphate buffer (1 ∶ 9,V/V,pH 6.0) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 280 nm and column temperature was room temperature.RESULTS: The linear range of NA was 0.5～40 ?g?L-1 (r=0.999 9) with an average recovery of 97.49% (RSD